STEC Testing - Real-Time BAX® System detection

BAX® System Real-Time PCR STEC Suite

ned with the USDA FSIS approach, the STEC screening assay detects virulence genes stx and eae to clear negative samples quickly and cost-effectively. Using the same lysate, panel assays determine if positive screening samples contain one of the top six serogroups of non-O157 Shiga toxin-producing E. coli.

Real-time PCR STEC Suite


Part #:

D14642964 - STEC Screening

D14642970 - STEC Panel 1

D14642987 - STEC Panel 2


stx and eae genes - STEC Screening

E. coli O26, O111, O121 - STEC Panel 1

E. coli O45, O103, O145 - STEC Panel 2

Matrices:   Raw ground beef, beef trim, produce
Enrichment time:   9-12 hours, depending on food type, sample volume and enrichment media
PCR time:   55 minutes
Detection level:   10^4 cfu/mL, after enrichment


USDA FSIS - MLG 5B.05 for meat products, carcass and environmental sponges

Certifications and Approvals

AOAC Research Institute - Performance Tested Method #091301 - validated on raw ground beef, ground beef with soy, and beef trim

BAX® is a registered trademark of DuPont for its line of equipment, reagents and software used to analyze samples for microbial contamination. BAX® System Q7 instruments are sold under licensing arrangement with Applied Biosystems for food testing. Use for research and development, quality assurance and quality control testing under supervision of technically qualified persons. Not approved for clinical use. Please read the limitation of warranty and liability before use.