E. coli Testing - Lateral Flow System

DuPont™ Lateral Flow System for E. coli O157

Rapid antibody-based alternative to culture methods

LFS for E. coli testing

The DuPont™ Lateral Flow System (LFS) for E. coli O157 uses test strips that employ a unique combination of anti-E. coli O157antibodies and colloidal gold conjugate coated on the surface of a membrane. After enrichment, drop the test strip into an aliquot of enriched sample broth, and read the results 10 minutes later. One control line is built into the lateral flow strip so you know the test has worked correctly. Two lines indicate a positive result.

Enrichment Media

LFS E. coli enrichment media reduces enrichment time to 8 hours for 25-g samples or 10-15 hours for 375-g samples. The media powder is mixed directly with sterile water for immediate use. After enrichment, you can choose to heat-treat the samples or test them immediately.

Applications

This LFS test detects E. coli O157, including O157:H7, in raw ground beef and boneless beef trim.

Approvals

AOAC-RI Performance Tested Method #010601 - validated on ground beef an boneless beef to perform as well as the reference culture method, even at low spike levels (1-4 cfu/25g sample)  

Please note: LFS for E. coli O157 is available outside the U.S. only.

Use for research and development, quality assurance and quality control testing under supervision of technically qualified persons. Not approved for clinical use. Please read the limitation of warranty and liability before use.