DuPont™ AmberLite™ XAD™ 7HP Polymeric Adsorbent Resins

 
 
 

DuPont™ AmberLite™ XAD™ polymeric adsorbent resins are nonionic, macroporous crosslinked resins that selectively adsorb molecules based on a combination of hydrophobic interactions and molecular size.  These resins can be used in single-use adsorption mode to capture impurities from products streams for disposal, or they can be regenerated and used in an adsorption/desorption mode to separate products and impurities across many production batches. 

DuPont™ AmberLite™ XAD™ 7HP is a crosslinked aliphatic acrylic adsorbent resin with water retention capacity of 61-69%, average surface area of 750 m2/g, and a mean pore size of ~550 Å.  Due to its aliphatic nature, AmberLite™ XAD™7HP can adsorb non-polar compounds from aqueous systems and can also adsorb polar compounds from non-polar solvents.  See Product Data Sheet for more information.

DuPont offers a range of XAD™ products of varying chemistries, particle sizes, surface areas, average porosities, and pore sizes.  Products are sold as free-flowing wet resin beads.  The main product characteristics to consider when selecting an adsorbent resin for bioprocessing are the following:

• Particle Size: Particle size impacts kinetics and backpressure. Smaller particles will give faster kinetics
  but will have higher backpressures.
• Surface Area: Surface area impacts operational capacity and kinetics, with higher surface area giving 
  higher operational capacity and faster kinetics.
• Porosity: Porosity is related to the surface area. Particles with high porosity will have more surface area
  for the molecules to interact with, provided the pore size is large enough to accommodate
  the molecule. Therefore, higher porosity will generally lead to higher operational capacity.
• Pore Diameter: Pore diameter impacts the degree of separation and molecule recovery. 
  Large pores will allow better recovery of large molecules.
• Chemistry: The chemistry of the resin (DVB vs acrylic) impacts how tightly molecules are held. 
  Hydrophobic molecules will be more loosely held on acrylic particles, making it easier to 
  desorb the bound molecules and reducing the amount of solvent required in the desorption step.
 
 
 
 
 
 
 
 
 
 
 
 

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