DuPont™ AmberLite™ XAD™ 7HP Polymeric Adsorbent Resins
DuPont™ AmberLite™ XAD™ polymeric adsorbent resins are nonionic, macroporous crosslinked resins that selectively adsorb molecules based on a combination of hydrophobic interactions and molecular size. These resins can be used in single-use adsorption mode to capture impurities from products streams for disposal, or they can be regenerated and used in an adsorption/desorption mode to separate products and impurities across many production batches.
DuPont™ AmberLite™ XAD™ 7HP is a crosslinked aliphatic acrylic adsorbent resin with water retention capacity of 61-69%, average surface area of 750 m2/g, and a mean pore size of ~550 Å. Due to its aliphatic nature, AmberLite™ XAD™7HP can adsorb non-polar compounds from aqueous systems and can also adsorb polar compounds from non-polar solvents. See Product Data Sheet for more information.
DuPont offers a range of XAD™ products of varying chemistries, particle sizes, surface areas, average porosities, and pore sizes. Products are sold as free-flowing wet resin beads. The main product characteristics to consider when selecting an adsorbent resin for bioprocessing are the following:
| • Particle Size: | Particle size impacts kinetics and backpressure. Smaller particles will give faster kinetics |
| but will have higher backpressures. | |
| • Surface Area: | Surface area impacts operational capacity and kinetics, with higher surface area giving |
| higher operational capacity and faster kinetics. | |
| • Porosity: | Porosity is related to the surface area. Particles with high porosity will have more surface area |
| for the molecules to interact with, provided the pore size is large enough to accommodate | |
| the molecule. Therefore, higher porosity will generally lead to higher operational capacity. | |
| • Pore Diameter: | Pore diameter impacts the degree of separation and molecule recovery. |
| Large pores will allow better recovery of large molecules. | |
| • Chemistry: | The chemistry of the resin (DVB vs acrylic) impacts how tightly molecules are held. |
| Hydrophobic molecules will be more loosely held on acrylic particles, making it easier to | |
| desorb the bound molecules and reducing the amount of solvent required in the desorption step. |
Uses
- Capture or recovery of small molecule drugs (e.g., antibiotics, enzymes, proteins)
- Removal of non-aromatic compounds from polar solvents
- Capture/concentrate of plant or animal extracts
- General adsorption of organics from polar and non-polar streams (e.g., polyphenol extraction)
Advantages
- Excellent chemical and thermal stability
- High surface area
- Aliphatic functionality for adsorbing compounds of varying degrees of polarity